In Silico Restriction Enzyme Digests to Minimize Mapping Bias in Genomic Sequencing

Department of Melanoma Medical Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA; Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA; MTA-DE Momentum, Genome Architecture and Recombination Research Group, Research Centre for Molecular Medicine, University of Debrecen, Nagyerdei krt 98, Debrecen 4032, Hungary; Department of Biochemistry and Molecular Biology, University of Debrecen, Egyetem sq. 1, Debrecen 4032, Hungary Correspondence: Lóránt Székvölgyi, PhD, Department of Biochemistry and Molecular Biology, University of Debrecen, Nagyerdei krt. 98, Debrecen 4032, Hungary. E-mail: [email protected] A commonly used genome fragmentation method in next generation sequencing, restriction endonuclease (RE) digestion, may severely compromise genomic mapping resolution and prevent the functional annotation of certain chromosomal regions unless REs are applied in correct combinations to sample all genomic regions with an equal probability.